Colonial morphology varies considerably, sometimes even within the same culture. The colonies are small to medium sized and typically grey to grey-yellow and translucent. Some are smooth and dome-shaped with an entire margin, whilst others are rough with a lobate margin, still others are flat with an irregular surface and a filamentous margin.
Clostridium perfringens is non-proteolytic and is not associated with a distinct odour.
This culture has been incubated anaerobically.