النتائج (
العربية) 1:
[نسخ]نسخ!
.Restriction digestion of the gene and the vector Here the vector to be used is pET28a and pMD18-T vector. -Then both the vector-And the gene insert is incubated with the respective restriction enzymes for the desired timeAfter the restriction digestion the vector and the gene so digested is run in the agarose gel electrophoresis for purification purpose. -The band showing the corresponding vector band and the gene band is cut and then eluted by the help of the gel extraction kit
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